If you have a page Default.aspx on your web root, and your pictures are in a folder named "images" then you should just be able to set src="images/image.jpg" (without the drive letter). If that's not working we need more details about the problem. What type of page (HTML or ASPX) contains your image? What tag (<img>, <asp:image>) are you using to display the image?
There was an ASP.NET security patch released a few months ago which prevented images from displaying when the SRC attribute contained "..\", or ending with a trailing slash, so make sure you aren't using that nomenclature.
-Dan
This posting is provided "AS IS" with no warranties, and confers no rights.